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Limitations of Macroscopic Fluorescence Imaging for the Estimation of Tumour Growth in an Orthotopic Glioma Mouse Model

Publication at First Faculty of Medicine |


Imaging methods based on light detection are being increasingly used for assesment of tumor growth in animal models. There are no studies assesing macroscopic FLI for monitoring of tumor growth in glioma mouse model.

Human glioma cells expressing mKate2 were implanted to the brain of imunodeficient mouse. The tumor size was estimated by FLI and volume determined morphometrically.

The fluorescent signal did not significantly correlate with the tumor volume. Therefore planar fluorescence imaging appearas to be suitable for semi-quantitative assesment of tumor growth rather then for precise volume estimation of xenografts located deep within the brain tissue.