Publication at First Faculty of Medicine |
2009
Abstract
An indirect competitive enzyme immunoassay of Enterobacter sakazakii has been developed. The rabbit polyclonal antibodies to heat-labile or heat-stable antigen of the type strain E. sakazakii CNCTC 5739(T) were prepared for these purposes.
The detection limits of enzyme immunoassays were within the range 0.6-14.4 x 10(5) cells/ml. Antibodies raised to heat-labile antigen were serotype-specific.
Although they contain non-specific IgG fractions binding periplasmatic and cytosol proteins, the interactions of these immunoglobulins are not manifested under conditions of ELISA developed.