Determination of S-Adenosylmethionine and S-Adenosylhomocysteine by LC-MS/MS and evaluation of their stability in mice tissues

Abstract

We present a rapid and sensitive LC-MS/MS method for SAM and SAH determination in mice tissues. The method is based on chromatographic separation on a Hypercarb column filled with porous graphitic carbon stationary phase.

Sufficient retention of SAM and SAH on the chromatographic packing allows simple sample preparation protocol avoiding solid phase extraction step. No significant matrix effects were observed by analysing the tissue extracts on LC-MS/MS.

Keywords

• S-Adenosylmethionine
• S-Adenosylhomocysteine
• Mass spectrometry
• Liquid chromatography
• Mouse tissues
• cystathionine beta-synthase
• performance liquid-chromatography
• tandem mass-spectrometry
• adenosyl-l-methionine
• plasma homocysteine
• cerebrospinal-fluid
• methylation ratio
• deficient
• mouse
• hydrolase