A rapid separation of two distinct populations of mouse corneal epithelial cells with limbal stem cell characteristics by centrifugation on percoll gradient
2008
Abstract
This study showed that there are two distinct populations of corneal epithelial cells with SC characteristics (expression of ABCG2 and Lgr5, an SP phenotipe) that can be isolated from the mouse limbus. Percoll gradient centrifugation is a convenient method of enriching and harvesting such cells for further study.