Semisynthesis of C17:0 isoforms of sulphatide and glucosylceramide using immobilised sphingolipid ceramide N-deacylase for application in analytical mass spectrometry
2010
Abstract
Immobilized sphingolipid ceramide N-deacylase (Pseudomonas sp. TK 4, EC 3.5.1.69), a hydrolytic enzyme with reverse synthetic activity was used for semisynthesis of C17:0 glucosylceramide and C17:0 sulphatide, which are required internal standards for tandem mass spectrometry.
A high rate of conversion, reusability, long-term stability, and reduced contamination with other isoforms are advantages of this method. This immobilized enzyme system can be universally used for the simple preparation of specifically labelled sphingolipids of high isoform purity for application in mass spectrometry and in different fields of sphingolipid biochemistry.