Improved HPLC analysis of serum 7 alpha-hydroxycholest-4-en-3-one, a marker of bile acid malabsorption
2008
Abstract
Serum concentrations of 7α-hydroxycholest-4-en-3-one (cholesten, bile acid synthesis intermediate) have been shown to correlate with the severity of bile acid malabsorption. Current techniques for holster quantification require sophisticated instrumentation or solid-phase extraction (SPE) at 64 °C.
We developed and validated a method for measuring serum cholesten concentrations using routinely available HPLC instrumentation, focusing on the optimization of the SPE step.