Od krystalové a NMR struktury, footprintů a cryo-electron-mikrogramů k velkým a ohebným strukturám: nanoškálové modelování nukleozomální stopky
2011
Abstrakt
By combining (i) crystal and NMR structures of the nucleosome core particle and H1, (ii) the known nano-scale structure and elasticity of DNA, (iii) footprinting information on the location of protected sites on the DNA backbone and (iv) cryo-electron micrographs of reconstituted tri-nucleosomes, we arrive at a description of a polymorphic, hierarchically organized stem with a typical length of 20 +/- 2 base pairs.