Properties of endoproteinase Glu-C immobilized to cellulose magnetic particles

Abstract

The simple procedure for coupling of Staphylococcus aureus endoproteinase GluC (V8 protease) to divinyl sulfone-activated cellulose magnetic beads was elaborated. The immobilized proteinase was shown to be suitable for the preparation of proteolytic digest suitable for the following MALDI-TOF MS analysis of peptides.

Keywords

• enzyme immobilization
• immobilized endoproteinase Glu-C
• magnetic bead cellulose
• alpha-casein
• bovine serum albumin