Nonpathogenic Entamoeba dispar quickly outgrows pathogenic Entamoeba histolytica in mixed xenic cultures

Abstract

Entamoeba histolytica and Entamoeba dispar are two microscopically indistinguishable amoebae living in the human colon. The former is a pathogen, whereas the latter is a non-pathogenic commensal.

Using a model system of in vitro cocultures and PCR detection of the Entamoeba species, we found that the non-pathogenic species can rapidly outgrow the pathogen in xenic cultures.

Keywords

• diagnosis
• entamoeba
• in vitro culture
• PCR
• protozoa