Publication at First Faculty of Medicine |
2011
Abstract
Mesenchymal stromal cells (MSC, MSCs) are cells firstly described 35 years ago in bone marrow but present basically in all adult and fetal tissues, where they reside in the vessel wall as part of the population of pericytes. In the vessel wall as part of the population of pericytes.
These rare cells (10-6-10-4 of nucleated cells in various tissues received special attention of biomedical researchers as they are easy to expand and able to differentiate in various cell and tissue types. Later, these cells were found to be little immunogenic and to have immunosuppressive properties, which they exert by action on T cells, B cells, NK cells and dendritic cells ...
Keywords
Mesenchymal stromal cells (MSC, MSCs) GMP preparation airborne particulate cleanliness. autologous / allogeneic material bone marrow mononuclear cells Trophoblastic tissues placental MSCs monoclonal antibodies cultivation medium research-grade media DMEM fetal calf serum allergic reactions xenogeneic protein serum-free media autologous human plasma cytokine-suplemented medium antibiotics Harvesting of adherent cells cryopreservation viability clonogenicity senescence Donor screening Microbial contamination Mycoplasma Endotoxin testing Tumorigenicity