Rapid and easy method for monitoring oxidative stress markers in body fluids of patients with asbestos or silica-induced lung diseases
2009
Abstract
Sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers in body fluids (exhaled breath condensate, plasma and urine): 8-iso-prostaglandin F-2 alpha, malondialdehyde and 4-hydroxynonenal and the detection method LC-ESI-MS/MS. The method was tested on samples obtained from patients diagnosed with asbestosis, pleural hyalinosis or silicosis, i.e. occupational lung diseases caused by fibrogenic dusts, inducing oxidative stress in the respiratory system, and then compared to samples from healthy subjects.