The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method
Abstract
Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies.
Conventionally used method for determination of T-lymphocytes proliferation, 3H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating, based on time limited acquisition of events and on its distribution according to forward and side scatter values, seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation.
We decided to compare these methods and determine mutual correlation and compatibility. Eleven patients were studied.
Both methods retained consistency with regard to survival and proliferation of lymphocytes. However, the correlation was not convincing.
Therefore, both methods might be used for evaluation of MLR, but each of them gives specific and complementary information