Application of the new insertion-deletion polymorphism kit for forensic identification and parentage testing on the Czech population
Abstract
We genotyped 55 unrelated Czech individuals. We also genotyped 11 trios to analyze DIPplex Kit (QIAGEN, Germany) suitability for parentage testing.
DIPplex Kit contains 30 diallelic autosomal markers. INDELs in DIPplex Kit were tested with linkage disequilibrium test, which showed that they could be treated as independent markers.
All 30 loci fulfill Hardy–Weinberg equilibrium. There were several significant differences between Czech and African populations, but no significant ones within European population.
Probabilityof a match in the Czech population was 1 in 6.8×1012; combined power of discrimination was 99.9999999999%.Average paternity index was 1.13–1.77 for each locus;combined paternity index reached about 27,000 for a set of 30 loci. The DIPplex kit is useful as an additional panel of markers in paternity cases when mutations in STR polymorphisms are present.
For application on degraded or inhibited samples, further optimization of buffer and primer concentrations is needed.