Abstract
Infection of orthopedic prosthesis is a major problem not only because of rapid formation of biofilms on the surface of the extraneous material replacements, but also for the difficult diagnosis. The impedance method was used to detect bacterial layer on the surface of micro-electrodes in vitro.
Then, the KB-12 sensor with spacing 15 µm was selected as the most suitable sensor. For experiments, Escherichia coli was chosen and the bacterial growth was measured during 12 hour intervals.
The impedance of the sensor KB-12 was changed by 10 percent when covered with a layer of bacteria.