Sesquiterpene lactone trilobolide activates production of interferon-gamma and nitric oxide

Abstract

Trilobolide (TB), a sesquiterpene lactone isolated from Laser trilobum is an inhibitor of sarco/endoplasmic reticulum Ca2+-ATPase (SERCA). We have found that upon the in vitro exposure to TB, rodent peritoneal cells and human peripheral blood mononuclear cells secrete high amounts of IFN-gamma.

The effect is associated with the stimulation of high output NO biosynthesis in rat cells. The stimulatory potential of TB depends on the activation of MAP kinases p38 and ERK1/2, and transcription factor NF-kappa B.

BAPTA-AM, a chelator of the intracellular calcium, remained without any effect on the secretion of IFN-gamma triggered by TB. These results demonstrate that TB is a potent immunostimulatory agent.

Keywords

• Trilobolide
• Sesquiterpene lactones
• SERCA inhibitor
• Interferon-gamma
• Nitric oxide
• Laser trilobum
• nf-kappa-b
• rat peritoneal-macrophages
• prostate-cancer cells
• murine macrophages
• tnf-alpha
• in-vivo
• raw264.7 macrophages
• tanacetum-parthenium
• inula-britannica
• gene-expression