Abstract
Carbapenemase-producing Enterobacteriaceae and Pseudomonas spp. are increasingly reported in many countries all over the world. Due to the resistance of those bacteria to almost all antibiotics (e.g. beta-lactams, aminoglycosides, fluoroquinolones), treatment options are seriously limited.
In the Czech Republic, the incidence of carbapenemase-producing Enterobacteriaceae seems to be low, restricted to only three cases detected between 2009 and 2010. Here, we describe molecular typing of 15 carbapenemase-producing Klebsiella pneumoniae isolates identified in the Czech Republic during 2011.
Five VIM-1-producing isolates belonging to sequence type (ST) 11 and one VIM-4-producing isolate of ST1029 have been detected. blaVIM-1 and blaVIM-4 as a part of class 1 integrons were chromosomally located or carried by a plasmid belonging to A/C replicon type (blaVIM-4). KPC-3-producing isolates of ST512, recovered from six patients, caused an outbreak.
Three more isolates producing KPC-2 enzyme belonged to ST258. Both blaKPC genes were part of the Tn4401a transposon carried on plasmids of the pKpQIL type.
The isolates were resistant to all antibiotics tested except colistin and/or gentamicin. Four of these 15 strains were recovered from patients repatriated to the Czech Republic from Greece and Italy.
This is the first report of outbreaks caused by carbapenemase-producing Enterobacteriaceae in the Czech Republic.