HPLC method to determine the residual concentration of estrone during mouse sperm capacitation in vitro

Abstract

High performance liquid chromatography with UV detection determines the status of estrone during mouse sperm capacitation in vitro. The method enables to ascertain a free estrone, and the estrone bound to the bovine serum albumin in capacitation medium.

Keywords

• HPLC
• endocrine disruptors
• sperm
• estrone
• capacitation