Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling

Abstract

Enzymatic construction of single-nucleobase redox-labelled oligo-nucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magneto-separation and the second PEX with a full-length template.

Keywords

• cross-coupling reactions
• DNA-protein interactions
• nucleoside triphosphates
• enzymatic incorporation
• functionalized DNA
• restriction endonucleases
• electrochemical detection
• primer extension
• nucleotides
• construction