Intra-specific rDNA-ITS restriction site variation and an improved protocol to distinguish species and hybrids in the Daphnia longispina complex

Abstract

We improved the recently developed protocol for identification of species and hybrids of the Daphnia longispina complex based on restriction fragment length polymorphism (RFLP) of the internal transcribed spacer region (ITS).

Keywords

• Daphnia longispina species complex
• ITS-RFLP
• ribosomal DNA (rDNA)
• ITS
• intragenomic
• interspecific hybrids
• ribosomal dna
• intragenomic variation
• sexual reproduction
• internal transcribed spacer
• differentiation
• hybridization
• systematics
• evolution