Histological assessment of tissue from large human bone defects repaired with β-tricalcium phosphate
2014
Abstract
This report describes the histological characteristics of large human bone defects that were implanted with β-tricalcium phosphate (β-TCP). Samples were obtained longer after the primary operation than in the earlier studies.
We assessed a total of nine biopsies taken 33-208 weeks after implantation. The tissue sections were stained with hematoxylin-eosin for general observation, with Gomori stain to visualize the reticulin fibers, and with an antibody against tartrate-resistant alkaline phosphatase (TRAP) to characterize the cells.
Ongoing bone remodeling was observed even 208 weeks after implantation as determined by the presence of osteoclasts and active osteoblasts and new woven and lamellar bone.