Abstract
We isolated and expanded 16 lines of DPSCs in three different media over 40 population doublings. DPSCs cultivated in all media were cytogenetically stable and showed no signs of spontaneous differentiation.
Over the entire cultivation period, we observed no changes in cell viability. Medium containing 2 % FCS supplemented with ITS provided better cultivation conditions for DPSCs (shorter DT and more stable proliferation activity were measured) than other tested media (e.g. most cited 10 % FCS or high FCS concentration media).
Decrease in concentration of FCS and adding ITS into media had no negative effects on basics biological characteristics(viability, cell diameter). DPSCs cultivated in 2 % FCS with ITS showed lower positivity mesenchymal stem cell markers and HLA I compared to DPSCs cultivated in 2 % FCS or 10 % FCS media.