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Expression of cytoskeletal proteins in hepatic stellate cells isolated from normal and cirrhotic rat liver

Publication at Central Library of Charles University, Faculty of Medicine in Hradec Králové |
2005

Abstract

Hepatic stellate cells (HSC) are located in Disse spaces of normal rat liver. In fibrotic liver they become activated, proliferate and they undergo transdifferentiation into myofibroblast-like cells.

Changes in the cell phenotype are accompanied by changes in the cellular cytoskeleton. We have studied the expression of alpha-smooth muscle actin and intermediate filament proteins vimentin, desmin and glial fibrillary acidic protein (GFAP) by immunocytochemistry in HSC cultured for 2 or 7 days after isolation.

HSC were isolated from normal or CCl4-cirrhotic rat liver.