Insights into the oxidative stress response in Frahcisella tularensis LVS and its mutant Delta iglC1+2 by proteomics analysis

Abstract

The aim of this study was to find and identify proteins of F. tularensis live vaccine strain induced in the presence of hydrogen peroxide, and to investigate the role of the IglC protein in the regulation of genes expressed upon peroxide stress. The [35S]- radiolabelled protein patterns were examined for both the wild live vaccine strain and its ?iglC1 + 2 mutant defective in synthesis of the IglC protein that was found to be strongly up-regulated during intracellular growth in murine macrophages in vitro and upon exposure to hydrogen peroxide.

Globally, we found 21 protein spots whose levels were significantly altered in the presence of hydrogen peroxide in both the wild-type and mutant strains.