Muscle, Liver and Kidney Interstitium Blood Flow Changes in Rats Measured by Microdialysis with Flow-marker Added
Abstract
Microdialysis is a method appropriate for monitoring blood flow in interstitium of different tissues. The lower the output concentration of flow marker in microdialysis solution the higher tissue blood flow is.
We tried lithium and gentamicin as flow-marker instead of ethanol. The aim of study was to find out changes in the interstitial blood flow in selected tissues in rats before and after partial hepatectomy (PH) or nephrectomy (PN).
Male Wistar rats were divided into 4 groups. Gr. 1: microdialysis (MD) probe (CMA Microdialysis, Solna, Sweden) was inserted into skeletal muscle (in femur region) and into intact liver.
Gr. 2: MD probe was inserted into muscle and into liver remnant after 67% PH. Gr. 3: MD probe was inserted into muscle and intact kidney.
Gr. 4: MD probe was inserted into muscle and kidney remnant after 5/6 PN. Lithium and gentamicin concentration was then measured.
Our results indicate that gentamicin is not suitable as a flow-marker.