Prolonged agonist stimulation does not alter the protein composition of membrane domains in spite of dramatic changes induced in a specific signaling cascade.

Abstract

2-D electrophoresis of membr. domains (HEK293, S49 cells): long-term treatment with TRH or isoproterenol has no significant effect on the amount of any 150-170 major membr. domain protein, in spite of levels of the relevant G proteins markedly decreased.

Keywords

• trimeric G proteins
• membrane domains
• 2-D electrophoresis