PEG-Modified Macroporous Poly(Glycidyl Methacrylate) and Poly(2-Hydroxyethyl Methacrylate) Microspheres to Reduce Non-Specific Protein Adsorption

Abstract

To minimize non-specific protein adsorption on macroporous poly(glycidyl methacrylate) and poly(2-hydroxyethyl methacrylate) microspheres containing amino and/or carboxyl groups, the microspheres are coated with ,-bis-carboxy poly(ethylene glycol) and amino-terminated poly(ethylene glycol-co-propylene glycol) or -methoxy--amino poly(ethylene glycol). Adsorption of bovine serum albumin (BSA), -globulin, 125I-BSA, pepsin, and chymotrypsin on neat and PEGylated microspheres is determined by UVVIS spectroscopy of supernatants and eluates or by measurement of radioactivity in an ionization chamber.

Neat and PEGylated microspheres adsorb 0.870% and 0.0244% of protein, respectively.

Keywords

• coatings
• glycidyl methacrylate
• 2-hydroxyethyl methacrylate
• morphology
• poly(ethylene glycol)