Publikace na Přírodovědecká fakulta |
2013
Abstrakt
Immobilized polysaccharide-derived chiral stationary phases (CSPs) are a new type of chromatographic columns, which allow using much broader range of solvents. However, these columns are mostly used in normal phase HPLC mode.
In this study we worked in reverse phase mode and we used three CSPs that differed in the type of derivatization group or in the nature of the glycosidic linkage of the polysaccharide derivatives. The columns CHIRALPAK IA, CHIRALPAK IB and CHIRALPAK IC are based on tris-(3,5-dimethylphenylcarbamate) of amylose, tris-(3,5-dimethylphenylcarbamate) of cellulose and tris-(3,5-dichlorophenylcarbamate) of cellulose, respectively.