Comparison of Chiral Stationary Phases Based on Immobilized Polysaccharides in Reversed Phase Mode

Abstrakt

Immobilized polysaccharide-derived chiral stationary phases (CSPs) are a new type of chromatographic columns, which allow using much broader range of solvents. However, these columns are mostly used in normal phase HPLC mode.

In this study we worked in reverse phase mode and we used three CSPs that differed in the type of derivatization group or in the nature of the glycosidic linkage of the polysaccharide derivatives. The columns CHIRALPAK IA, CHIRALPAK IB and CHIRALPAK IC are based on tris-(3,5-dimethylphenylcarbamate) of amylose, tris-(3,5-dimethylphenylcarbamate) of cellulose and tris-(3,5-dichlorophenylcarbamate) of cellulose, respectively.

Klíčová slova

• enantiomers
• chromatographic columns