Determination of urinary 8-hydroxy-2'-deoxyguanosine in obese patients by HPLC with electrochemical detection
Abstrakt
A simple amperometric detector with a platinum tubular electrode was tested for the determination of urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) by high-performance liquid chromatography. Analyte was extracted from human urine using a single-step solid-phase extraction with the average recovery of 71+-7%.
Analysis was carried out on a C-18 analytical column under isocratic conditions. The limit of detection of 8-OHdG of 3 nM (S/N=3) was comparable with that achieved using the conventional amperometric detector.
Procedure was used for the determination of 8-OHdG levels in 10 obese patients (body-mass index>30 kg mMINUS SIGN 2) yielding the mean concentration 32+-27.1 nM of 8-OHdG or 1.85+-0.72 μmol 8-OHdG molMINUS SIGN 1 creatinine (4.63+-1.8 μg 8-OHdG gMINUS SIGN 1 creatinine), which is not much different from the levels found in healthy individuals.