Abstrakt
The proteasome plays a key role in the degradation of cellular proteins and therefore in the regulation of the cell cycle and the response to DNA damage, nutrients and other stimuli. The mature 26s proteasome dissociates under certain conditions to yield 19s regulatory particles (RPs) and proteolytically active 20s core particles (CPs).
In silico analysis of proteasomal transcription factor networks and mapping of transcription factor binding sites indicates a putative correlation between the differential expression of proteasomal CP and RP subunits and the differential expression of genes regulating cell cycle progression and genes regulating invasive growth. A further correlation is suggested, between the differential expression of CP alpha subunits, Pre9p and Pre6p (which can substitute for Pre9p) and the differential expression of genes regulating the cAMP-PKA pathway and negatively regulating dimorphic transition and genes mediating starvation and stress responses and positively regulating dimorphic transition.
These data suggest that yeast, like mammals, produce different types of proteasome under different conditions