5' end formation of the linear plasmid mRNAs

Abstrakt

We over-expressed ORF3p, coding for linear plasmid specific capping enzyme, as the N-terminal His-tagged fusion protein both in E. coli and baculovirus expression systems. Resulting capping enzyme was partially purified by Ni-NTA affinity chroma

Klíčová slova

• Vopálenský Václav
• 2 21 95 71 70 Kluyveromyces
• linear plasmids
• capping enzyme