Separation of twenty underivatized essential amino acids by capillary zone electrophoresis with contactless conductivity detection
Abstrakt
Twenty underivatized essential amino acids were separated using capillary zone electrophoresis and consequently detected with contactless conductivity detection (CCD). A simple acidic background electrolyte (BGE) containing 2.3 M acetic acid and 0.1% w/w hydroxyethylcellulose (HEC) allowed the electrophoretic separation and sensitive detection of all 20 essential amino acids in their underivatized cationic form.
The addition of HEC to the BGE suppressed both, electroosmotic flow and analyte adsorption on the capillary surface resulting in an excellent migration time reproduci- bility and a very good analyte peak symmetry. Additionally, the HEC addition signifi- cantly reduced the noise and long-term fluctuations of the CCD baseline.
The opti- mized electrophoretic separation method together with the CCD was proved to be a powerful technique for determination of amino acid profiles in various natural samples, like beer, yeast, urine, saliva, and herb extracts.