Influence of Metals onto sIgA, IgA1 and IgA2 Production in Saliva and Cytokine Production by Lymphocytes in Patients Undergoing Implantation Therapy in Dentistry
Abstract
The purpose of the study was to compare sIgA, IgA1 and IgA2 production in saliva before and after implantation therapy and to compare in vitro cytokine production by stimulated lymphocytes in patients undergoing implantation therapy in dentistry. Saliva from 21 patients was collected before implantation therapy and saliva from 9 patients after therapy and sIgA, IgA1 and IgA2 antibody production was established using RID method.
Lymphocytes from 15 patients were stimulated by mercury and titanium antigens and production of 39 cytokines was established using Quantibody INF-3 array. Patients which reacted to titanium antigen and therefore zirconia implant was used in these patients, we found non-significant increase in sIgA, IgA1 and IgA2 production in saliva after implant therapy.
On the other hand, in patients tolerating titanium, we didn´t find any differences in sIgA, IgA1 and IgA2 production before and after implantation. When compared to non-stimulated lymphocytes, lymphocyte culture stimulated by mercury antigen produced significantly increased levels of eotaxin-2, MIP-1a and MIP-1b, lymphocyte culture stimulated by titanium antigen produced significantly increased levels of IL-1ra and significantly decreased levels of IL-10.