Comparison of cytokine production in human lymphocytes and human tissue culture THP 1 stimulated by metalic ions
Abstract
Introduction: Physiological interaction of foreign materials (including metallic ions) with oral mucosal tissue can lead to oral tolerance induction. However, a minority of humans induce an intense reaction to metal dental reconstruction.
Cytokines produced during the interaction of lymphocytes and macrophages play an important role in activation and regulation of immune response. Aim and methods: Our study aimed at exploring cytokine production in lymphocytes of mercury and nickel hypersensitive patients (verified with a test of lymphocyte proliferation) and in a monocyte-macrophage cell line of human origin THP 1.
In all cases, the cells were cultivated for five days with an addition of mercury and nickel salts. Cytokines in culture media were determined by Ray Bio® Human Inflammation Antibody Array III and compared with results of control group cells, cultivated without addition of metallic salts.
Results: Lymphocytes of hypersensitive patients predominantly produced pro-inflammatory cytokines, for example IL-1 alpha, IL-11, IL-17, IFN-gamma, TNF-beta, sTNFRI, sTNFRII and TIMP-2. These cytokines were observed in both cases of metallic salts stimulation.
On the other hand, cell culture THP 1 predominantly produced anti-inflammatory cytokines after stimulation with both metallic salts. The results suggested that in the case of hypersensitivity diagnosis, to follow the reaction of both lymphocytes and monocyte-macrophage cells should be recommended.