Abstrakt
Capillary electrophoresis is widely used separation technique, which is very well described theoretically. Existing theoretical models offer deep insight into electrophoretical separation and enable to predict the results of separation, and thus, to propose the best separation conditions.
Recently, our group presented the complete theoretical model of electromigration for separation systems with complexation agents, which was implemented into our simulation program Simul 5 Complex. In this paper Simul 5 is verified for separation systems with weak electrolyte analytes and neutral chiral selectors.
The simultaneous effect of complexation and dissociation is in the scope of interest. The results of the simulations are compared with theoretical predictions and experimental data found in literature.
Very good capability of the model to predict otherwise unexpected phenomena such as electromigration dispersion or reversal of migration order of enantiomers is demonstrated.