Apparent presence of Ser133-phosphorylated cyclic AMP response element binding protein (pCREB) in brain mitochondria is due to cross-reactivity of pCREB antibodies with pyruvate dehydrogenase

Abstract

Examination of subcellular localization of transcription factor CREB in rat brain. With mitochondria cross-reactivity of commercial antibodies directed against Ser133-phosphoCREB with pyruvate dehydrogenase was found, rather than mitochondrial localization of authentic CREB