Abstract
Solid amalgam electrodes represent a suitable alternative to mercury electrodes due to their similar electrochemical properties and non-toxicity of the amalgam material. In this contribution the silver solid amalgam electrode in three modifications (mercury meniscus modified, polished, and mercury film modified) was employed as indicator electrode in "wall-jet" amperometric detection cell for HPLC determination of carmustine, an antineoplastic chemotherapy drug containing reducible N-nitroso group.
Under optimized conditions (mobile phase BR buffer (pH 3.0) / methanol (60/40; v/v); flow rate 1 mL min-1; detection potential -1.2 V; injection volume 25 µL), limits of quantitation of ~ 1.5.10-5 mol L-1 were achieved for all types of tested silver solid amalgam electrode. The repeatability of the detector response is satisfactory.