Preparation of a biologically active apo-cytochrome b5 via heterologous expression in Escherichia coli

Abstract

The results of this work describe for the first time a novel procedure of heterologous expression of apo-cytochrome b5, its purification and reconstitution with heme into the holoprotein form, which is fully active to modulate the CYP-catalyzed reactions.

Keywords

• Liver microsomal cytochrome-b5
• rat-liver
• electron-transfer
• endoplasmic-reticulum
• apocytochrome b(5)
• reductase system
• p-450 reductase
• p450 reductase
• heme-binding
• membrane