Molecular Characterization of Clinical Isolates of Human Immunodeficiency Virus Resistant to the Protease Inhibitor Darunavir
2009
Abstract
In this work we report structural and enzymological analysis of a panel of clinically derived mutant HIV-1 proteases with high levels of phenotypic resistance to darunavir. Recombinant virus phenotypic characterization, enzyme kinetics, and X-ray structural analysis are used to explain darunavir resistance development in HIV-positive patiens.