Effect of intermittent incubation and clutch covering on the probability of bacterial trans-shell infection
Abstract
Microbial infection is considered a critical cause of hatching failure in birds. Although several behavioural mechanisms are believed to improve reproductive success in birds, their direct effect on the risk of bacterial trans-shell infection (BTSI) remains to be tested.
Here, we focus on the protective roles of intermittent incubation and covering of the clutch with nest lining during the laying period, when eggs are highly susceptible to BTSI. To our knowledge, this study is the first to use culture-independent PCR-based methods to measure quantitative and qualitative indices of BTSI.
In our experiment, we exposed Mallard Anas platyrhynchos eggs that were either intermittently incubated (I-INCUB) or un-incubated (I-UNINCUB), and covered (COV) or uncovered (UNCOV) with nest lining. Hatchability of I-INCUB eggs was twice that for I-UNINCUB eggs.
The presence and degree of BTSI had no effect on hatching success of experimental eggs. The residual weights of ducklings hatched from infected and I-INCUB eggs were lower than those from uninfected and I-UNINCUB eggs.
In addition, ducklings originating from COV eggs were heavier than those hatched from UNCOV eggs. Intermittent incubation and clutch covering had no effect on the probability or degree of BTSI.
Although the effect of BTSI is considered less detrimental in temperate birds, we show that the presence of BTSI inside the egg may significantly affect hatchling phenotype. This represents a novel insight into the role of BTSI in the reproductive success of birds and implies that the use of molecular PCR-based methods is required in future studies for a better understanding of such causality.