Aim of this study was an evaluation of the novel triplex polymerase chain reaction (PCR) assay for the analysis of polymorphic Y-chromosomal short tandem repeat loci (Y-STR). We focused on the comparison of the genetic diversity between the Y-STR extended haplotype loci and that of the 3 additional loci, and on the benefit of using DYS449, DYS456 and DYS458 in forensic and population genetics applications.
Population comparison showed no difference between Czech, Bavarian, Austrian and Saxon sample set. Minor difference was found between Czech and Polish sample set. 3 Y-chromosomal microsatellite polymorphisms typing may provide a useful complement to already established sets of Y-STRs.