Allele loss in Wilms tumors of chromosome arms 11q, 16q, and 22q correlates with clinicopathological parameters
Abstrakt
An extended analysis for loss of heterozygosity (LOH) on eight chromosomes was conducted in a series of 82 Wilms tumors. Observed rates of allele loss were: 9.5% (Ip), 5% (4q), 6% (6p), 3% (7p), 9.8% (11q), 28% (11 p 15), 13.4% (16q), 8.8% (18p), and 13.8% (229).
Known regions of frequent allele loss on chromosome arms Ip, I I p 15, and 16q were analyzed with a series of markers, but their size could not be narrowed down to smaller intervals, making any positional cloning effort difficult. In contrast to most previous studies, several tumors exhibited allele loss for multiple chromosomes, suggesting an important role for genome instability in a subset of tumors.
Comparison with clinical data revealed a possible prognostic significance, especially for LOH on chromosome arms I Iq and 229 with high frequencies of anaplastic tumors, tumor recurrence, and fatal outcome. Similarly, LOH 16q was associated with anaplastic and recurrent tumors.
These markers may be helpful in the future for selecting high-risk tumors for modified therapeutic regimens.