Publication at Second Faculty of Medicine |
1996
Abstract
Collagen type-I CNBr peptides were separated under acidic conditions by capillary electrophoresis. Separation conditions were: 100 mM phosphate buffer pH 2.5, 50 cmx50 mu m capillary (placed in a cartridge), 8 kV, running time 30-45 min, detection by UV at 200 nm.
The peptides were separated strictly by their molecular mass and the overall pattern was well comparable to RP-HPLC separations of these analytes. It is proposed that the separation mechanism may involve hydrophobic sorptions to the capillary wall.