Publication at Third Faculty of Medicine |
2000
Abstract
Oxidative stress is an important factor in many pathological and toxicological processes. To follow-up the oxidative stress it is possible to determine the products resulting from free radical damage, these include : lipid peroxidation products, DNA-hydroxylation products and protein hydroxylation products. 8-hydroxy- 2'-deoxyguanosine is hydroxylation product of DNA damage.
Its levels can be determined in urine or in tissues. The most sensitive method for the determination of 8-hydroxy-2'-deoxyguanosine is HPLC with electrochemical detection.