Determination of 8-hydroxy-2'-deoxyguanosine in untreated urine by capillary electrophoresis with UV detection
2003
Abstrakt
A capillary electrophoresis method with UV detection was developed for the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG) in untreated urine samples. The calibration graph for 8-OHdG in urine is linear in the concentration range 10-500 mg/l, and the detection limit is 5 mg/l (17 μM). 8-OHdG was determined in urine from oncological patients treated by radiation therapy.
Its concentrations relative to creatinine were found to be in the range 10-47 μg 8-OHdG/1 mg creatinine (4-19 μmol 8-OHdG/mmol creatinine). The overall time of the analysis of a urine sample was less than 15 min.