Abstract
Ex-vivo investigation of colorectal tissue by autofluorescence spectroscopy is reported. Wet microscopic mounts of healthy colon mucosa were examined and compared with those prepared from colon mucosa affected by adenocarcinoma.
The diagnosis of adenocarcinoma was verified by using clinical and histology means. Fluorescence spectra of tissue samples, excited by blue emitting semiconductor diode have been studied by using portable spectrometer with fixed grating and fibre optic system compatible with endoscope.
The study demonstrates differences in both the spectral shape and in the signal intensity in photoluminescence spectra emitted from tissue affected by adenocarcinoma as compared to that of healthy colon mucosa. The overall decrease and the shift of autofluorescence intensity to longer wavelengths have been observed for malignant tissue samples