Abstrakt
The structural changes and allergenicity of recombinant main celery allergen rApi g1 caused by the high pressure were studied. We have treated the buffer solutions of rApi g1 by high pressure at 500MPa for pressure holding times 10 and 20min and holding time temperatures 30 degrees C, 40 degrees C and 50 degrees C.
The structural changes were studied by circular dichroismus (CD) spectra. The allergenic reaction of the rApi g1 was tested by Western blot analysis.
The greatest changes of the structure were found at samples treated by 500MPa at 50 degrees C. The samples treated at this temperature at pressure levels 400, 450 and 500MPa and held for 10 and 20min showed that protein structure changes are positively correlated with pressure.
Western blot analysis evidenced that a pressure of 500MPa held for 10-20min at temperatures 30-50 degrees C did not change the allergenicity of the rApi g1 protein when compared with the untreated sample.