The effect of hippocampal aplication on antisense NR1/NR2 oligodeoxynucleotide on expression of postsynaptic density proteins and on prepulse inhibition
Abstract
N-methyl-D-aspartate receptor is widely examined in schizophrenia-related studies for last two decades. It has been shown that the glutamatergic system dysfunction is a complex phenomenon and so it might be important to focus the studies not only \NMDAR but also on associated molecules like proteins of postsynaptic density (PSD).
In our work, using it separately on in combination, antisense oligodeoxynucleotide (aODN) for NR1, NR2A and NR2B subunit of NMDAR, we affected expression of these proteins in rat hippocampus. We assessed prepulse inhibition of acoustic startle reaction (PPI) in rats and protein expression of NMDAR subunits and expression of PSD proteins.
There were significant differences in expression of PSD-95 and NR1 between groups. Application of aODN (NR2A, NR2B) was associated with a significant decrease of PSD-95.
PPI and expression of NR2A, NR2B and PSD-93 were not changed after aODN application