Determination of Midazolam by HPLC with UV detection and by GC with nitrogenphosphorus detector in rabbit plasma
2012
Abstract
A HPLC method with UV detection and GC method with nitrogen-phosphorus detector for determination of anesthetic midazolam were developed. The LOD for HPLC method is 3,4 ng/ml and for GC method is 0,34 ng/ml.
Repeatability of both methods are satisfactory for monitoring midazolam in plasma. Both methods were used for determination of midazolam in rabbit blood samples after nasal application (1 mg/kg).
Both methods give comparable results.