Too large intercontinental differentiation of Moina micrura (Crustacea: Anomopoda): One less cosmopolitan cladoceran?
Abstract
Existence of cryptic species complex within a cladoceran Moina micrura was demonstrated by mtDNA sequencing and hybridisation experiment. Moina micrura Kurz, 1874 (Crustacea: Anomopoda), commonly regarded as a cosmopolitan cladoceran species, can be found almost all over the world except for arctic and cold-temperate regions.
M. micrura has been recorded from virtually all types of limnetic habitats, including temperate permanent ponds, lakes, ephemeral desert pools, and tropical brackish fishponds. Its high morphological and ecological variability has so far been considered a result of intraspecific diversity.
We tested the hypothesis that genetically isolated groups exist within M. micrura with crossing experiment and DNA sequence analysis. We compared two clones originating from populations from Central Europe (area of the type locality) and Australia.
As there was extremely low production of hybrid eggs, the results of the crossing experiment strongly suggest the existence of reproductive isolation. The sequence divergence of mitochondrial gene for 12S rRNA (7.9%) was comparable to intraspecific differences within Daphnia pulex group.
The sequence divergence of the clones of Moina macrocopa from Europe and Central Africa was much lower (1.4%). Our results suggest that at least two sibling species exist within M. micrura complex, which is in agreement with other data questioning the cosmopolitanism of various cladoceran species.